WO2012119324A1

WO2012119324A1 - Intelligent wireless remote control stimulating device for animal memory training system

Info

Publication number: WO2012119324A1
Application number: PCT/CN2011/071870
Authority: WO
Inventors: 朱启文; 赵建文; 匡宝平; 王克威
Original assignee: 沈阳医学院
Priority date: 2011-03-07
Filing date: 2011-03-16
Publication date: 2012-09-13
Also published as: CN102160527B; CN102160527A

Abstract

An intelligent wireless remote control stimulating device for animal memory training system is provided, which can fundamentally solve the problem that the stimulating device for the existing animal memory training system is single, uses the electric stimulation-invasive stimulation which causes great side-effect on animals, and is inconvenient to operate. Said stimulating device includes an electric stimulation g module, a sound stimulation g module and a mechanical vibration stimulation module. The technical points are that the stimulating device also includes a wireless transmission module, a wireless receive module, and a relay drive module. The above modules are assembled and fixed on a carrier tied to the experimental animal body lateral. The wireless transmission module transmits a wireless signal according to a collected signal of experimental animal coming through a door. The wireless receive module determines a stimulating type and starts the relay drive module after receiving the wireless signal, and implements corresponding electric stimulation, sound stimulation, mechanical vibration stimulation or combined stimulation. The device is of reasonable design, wide accommodation, high control precision, and also has features such as simple circuits, easy production, convenient debugging and so on.

Description

Intelligent wireless remote control stimulation device for animal memory training system

[Technical Field]

The invention relates to a passive stimulation detecting device for researching and establishing an animal learning and memory behavior training method, in particular to a smart wireless remote control stimulation device for an animal memory training system. The invention belongs to biomedical engineering technology and can be applied to special stimulation devices in the fields of neurophysiology, neuropharmacology and psychology.

【Background technique】

At present, the study of animal behavior is an indispensable and important means to study advanced brain function and other neurosciences, especially in behavioral medical research such as neurophysiology and neuropharmacology. The mouse path training model and the automated behavior monitoring system are commonly used animal-specific experimental instrument models for studying the learning and memory function of rats (or mice) and screening for cognitive drugs.

There are many types of devices, most of which are single-stimulation---electrical, acoustic, optical, and mechanical vibration stimulation. Among them, electrical stimulation is an invasive stimulation that places the stimulating electrode in the animal. The side effects are large, and the operation is inconvenient. Therefore, the application range and effects are not satisfactory. The following compares and analyzes two similar patent devices as follows: 1. The "brain nerve electrical stimulation device with remotely controllable motion behavior" of the patent application number 200510047336 discloses a brain nerve stimulator, and the working process thereof is as follows: The cranial nerve stimulator sends an instruction, and then sends the command to the PC through the serial port to start the operation; at the same time, the cranial nerve stimulator sends a corresponding electrical pulse, which is stimulated by the implanted brain stimulation electrode. The device also includes a sound stimulator to assist the brain nerve electrical stimulator. The device has an invasive electrical stimulation to the animal, and thus has the disadvantage of being highly stimulating to the experimental animal and not easy to operate; 2. The device for non-invasive puncture of the animal body is disclosed in Patent Application No. 200780010744. The switch-controlled electric pulse stimulation device, although it is a non-invasive stimulation device, has a single function, and has a single performance in practical use, and cannot realize remote operation.

The "Roller Path Training Model and Brake Behavior Monitoring System" of the applicant's patent application No. ZL 2006 1 0045870.6 discloses a technical solution for applying acoustic, electrical and mechanical vibration stimulation to animals using a gate signal. It is to obtain stimulation information for the non-green channel set by the experimental mouse on the multi-channel maze, but it still cannot meet the intelligent requirements of the wireless remote control and needs to be improved.

[Summary of the Invention]

The object of the present invention is to provide a smart wireless remote control stimulation device for an animal memory training system, which fundamentally solves the single stimulation device of the existing animal memory training system, and the electrical stimulation - the invasive stimulation produces a larger animal Side effects, inconvenience to the problem.

The technical solution of the present invention is: a smart wireless remote control stimulation device for an animal memory training system, comprising an electrical stimulation, an acoustic stimulation, a mechanical vibration stimulation module, and the technical points thereof are: further comprising a wireless transmitting module, a wireless receiving module and a relay a driving module, the electrical stimulation, the acoustic stimulation, the mechanical vibration stimulation module, the wireless transmission module, the wireless receiving module, and the relay driving module are assembled together and fixed on a carrier bundled outside the body of the experimental animal, and the wireless transmitting module is collected according to the The pass signal of the experimental animal transmits a wireless signal, and after receiving the wireless signal, the wireless receiving module determines a stimulus type and activates the relay driving module to implement corresponding electrical stimulation, acoustic stimulation, and mechanical vibration on the experimental animal. Stimulate or combine stimulation.

The wireless transmitting module comprises a wireless remote control transmitting head IC1, a time base integrated circuit IC2, a diode D10, a potentiometer W, a manual switch K1, a Κ2, a Κ3 and a single chip IC7; the wireless receiving module comprises a wireless remote control receiving head IC3, and decoding Integrated chip IC4; the relay driving module comprises a single chip IC6, relays Ja, Jb, Jc _; The wireless transmitting module uses a single chip IC7 to control the time base integrated circuit IC2, and the diode D10 is a transmitting indicator light. When the P3.7 pin of the single chip IC7 outputs a high level, the corresponding pin of the time base integrated circuit IC2 is low. The time base integrated circuit IC2 is in a reset state, the wireless remote control transmitting head IC1 has no signal output, and the diode D10 indicator light is off; when the P3.7 pin of the single chip IC7 outputs a low level, the time base is integrated. The circuit IC2 is in a normal working state, and the wireless remote control transmitting head IC1 transmits N sets of wireless signals with time intervals, and the diode D10 indicator light is bright, ΝΞ≥1;

The wireless receiving module receives each set of wireless signals by using the wireless remote control receiving head IC3, and the single chip IC6 of the relay driving module identifies the selected stimulation type according to the number of times of the wireless signal, and further controls the corresponding relay Ja. , Jb, J _C normally open contacts to achieve stimulation.

The manual switches K1, Κ2, and Κ3 respectively correspond to acoustic stimulation, vibration stimulation, and electrical stimulation selection, and the single-chip microcomputer IC7 of the wireless transmission module controls the time base integration according to the setting states of the manual switches K1, Κ2, and Κ3. The circuit IC2 transmits a corresponding number of wireless signals.

The mechanical stimulation module includes a motor Μ, a resistor R 13 , and a normally open contact Kb controlled by a relay Jb. The resistor R13 and the normally open contact Kb are connected in series with the motor M, and are connected to the normally open contact Kb of the relay Jb and the ground. Meanwhile, when the normally open contact Kb of the relay Jb is closed, the motor M generates mechanical vibration stimulation.

The sound stimulation module includes a time base circuit IC5, resistors R7, R8, capacitors C3, C4, a normally open contact Ka controlled by the relay Ja, and a speaker Y. The capacitor C3 is connected between the pins 1 and 2 of the time base circuit IC5. The time base circuit IC5's 2 and 6 pins are shorted and then connected to the resistor R7. The resistor R7 is connected between the 6 and 7 pins of the time base circuit IC5. The resistor R8 is connected to the 4 and 7 pins of the 555 time base circuit IC5. Between the 4th and 8th pins of the time base circuit IC5, the normally open contact Ka controlled by the relay Ja is connected, and the capacitor C4 is connected in series with the speaker Y and connected between the 555 time base circuit IC5 and the ground. When the Ja-controlled normally open contact Ka is closed, the speaker Y emits a sound stimulus.

The electrical stimulation module comprises a normally open contact Kc controlled by a relay Jc, a transistor V5, a transformer T, a resistor R18, a light emitting diode D6, a diode D7-D9, a capacitor C5-C7 and an electrode DJ, and a collector connection of the transistor V5. The normally open contact Kc and the input end of the transformer primary winding L1 side, the base of the transistor V5 is connected to the other end of the transformer primary winding L1 through the resistor R18, and the emitter of the transistor V5 is connected to the transformer primary winding L2 terminal, the transformer times Side series diodes D7, D8, D9 and metal electrode DJ, diodes D7, D8 in series and capacitor C6 in parallel, diodes D8, D9 in series and capacitor C7 in parallel, diodes D8, D9, DJ in series with capacitor C5 in parallel, when When the normally open contact Kc controlled by the relay Jc is closed, the electrode DJ is electrically stimulated to the experimental animal.

The wireless receiving module further includes capacitors C8, C9, C10, and a resistor R9. The relay driving module further includes transistors V2, V3, and V4, diodes D3, D4, and D5, and resistors R10, R11, and R12. The positive and negative poles of the wireless remote control receiving head IC3 of the module are respectively connected to the positive and negative poles of the power source E, the negative pole of the wireless remote control receiving head IC3 and the speaker Y of the sound stimulation module, the capacitor C3, and the time base circuit IC5. 1. The capacitor C8, the capacitor C9, the capacitor C10, the pin 7 of the decoding integrated chip IC4, the collectors of the transistors V2, V3, and V4 of the relay driving module are grounded together, and the positive electrode of the wireless remote control receiving head IC3 is integrated with the decoding. The pin 4 of the chip IC4, the relay Ja, the relay Jb, the normally open contact of the relay Jc, the relay Ja, the relay Jb, the relay Jc coil and the transformer winding L1 of the electrical stimulation module are connected; wherein, the decoding integrated chip IC4 The 3 pin is connected to the wireless remote control receiving head IC3, and the 1 pin of the decoding integrated chip IC4 is grounded through the capacitor C8, and the 2 pin of the decoding integrated chip IC4 is grounded through the capacitor C9, and the integrated core is decoded. The 6 pin of IC4 is grounded through capacitor C10. The 7 pin of decoding integrated chip IC4 is directly grounded. The 6 pin of decoding integrated chip IC4 is grounded through resistor R9 and capacitor C10. P3. 1 and P3. 2 of single chip IC6 are connected. Enter the 8 pin of the decoding integrated chip IC4; PL 5 of the single chip IC6 is connected to the base of the transistor V2 through the resistor R10, the single chip IC6 The PI.6 is connected to the base of the transistor V3 through the resistor R11, and the P1.7 of the single chip IC6 is connected to the base of the transistor V4 through the resistor R12; the emitter of the transistor V2 is connected to the relay Ja, the relay Ja is anti-parallel diode D3, the transistor The emitter of V3 is connected to the relay Jb, the relay Jb is anti-parallel to the diode D4, the emitter of the transistor V4 is connected to the relay Jc, and the relay Jc is connected in parallel with the diode D5.

The wireless transmitting module comprises capacitors Cl, C2, diodes D1, D2, resistors Rl-R6, 15-R17, +12V regulated power supply connected to the wireless remote control transmitter IC1 positive pole, 555 time base circuit IC2 8 pins and resistors Rl, the resistor R1, the potentiometer W and the resistor R2 are connected in series, and the anti-series diode D2 is connected between the 2-pin of the time base circuit IC2 and the +12V regulated power supply, and the potentiometer W is connected to the time base circuit IC2 5 Pin, diode D1 is connected between time base circuit IC2, pin 5, pin 6, time base circuit IC2 6 pin and 2 pin ground through capacitor C 1 , time base circuit IC2 1 pin ground, time base circuit The 7 pin of IC2 is grounded through capacitor C2, the resistor R3 is connected to the 3-pin of time base circuit IC2 and the IN of wireless remote control transmitter IC1, and the negative pole of wireless remote control transmitter IC1 is grounded.

The carrier bundled on the outside of the body of the experimental animal is an elastic canvas, and the canvas is further provided with a stimulating electrode, and the two ends of the canvas are provided with a binding buckle.

The advantages and positive technical effects of the present invention are as follows: The present invention achieves non-invasive stimulation to animals, including three parts: acoustic stimulation, electrical stimulation, and mechanical vibration stimulation, and can perform acoustic, electrical, and mechanical vibration stimulation on animals or perform sound, electricity, and Single mode stimulation of mechanical vibration. In order to miniaturize the stimulation device, the stimulation device is divided into three detachable stimulation modules: acoustic, electrical and mechanical, for the user to select the stimulation solution. The device has reasonable design, wide adaptability and high control precision. A stimulus device that is effective for building complex cognitive memory models with effective real-time multiple stimulus outputs to meet the needs of more in-depth learning and memory behavior analysis. The transmitting-receiving circuit of the invention adopts a wireless remote control switch made by a micro wireless remote control transmitting and receiving device, and has the characteristics of simple circuit, easy manufacture and convenient debugging.

[Description of the Drawings]

The present invention is further described below in conjunction with the accompanying drawings:

Figure 1 is a front elevational view showing a specific structure of the apparatus of the present invention;

Figure 2 is a plan view of Figure 1;

Figure 3 is a rear elevational view of Figure 1;

Figure 4 is a schematic view showing the electrical principle of the device of the present invention;

Figure 5 is a diagram showing a wireless remote control transmitting circuit of the apparatus of the present invention;

6 is a flow chart of a program of the wireless control transmitting module of the present invention;

7 is a flow chart of a program of the wireless control receiving module of the present invention;

Figure 8 is a functional block diagram of the apparatus of the present invention;

Figure 9 is a schematic overall view of the device of the present invention tied to the body of a test mouse;

Figure 10 is a schematic view showing the specific structure of the labyrinth device;

Figure 11 is a plan view of Figure 10;

The serial number in the figure shows: 1 bonding buckle, 2 switch, 3 bonding buckle, 4 stimulating electrode, 5 box, 6 partition, 7 LCD screen, 8 motor, 9 mesh support net, 10 turntable, 11 water pipe, 12 Sink, 13 water brush, 14 bracket pulley, 15 brackets, 16 pulleys, 17 door holes, 18 food slots.

【detailed description】

The invention is applicable to an animal memory training system using a closed rotary maze, and is also a patent for the invention, a path training model for rats and braking. Behavior Monitoring System (Patent No.: ZL 2006 1 0045870.6) Designed and produced stimulation devices, as well as special instruments for research applications in other types of animal stimulation research.

First, the specific structure of the present invention is described in detail according to FIG. 1-9: The device is composed of an electrical stimulation module, a mechanical stimulation module, an acoustic stimulation module, a relay driving module, a wireless receiving module and a wireless transmitting module, and the above electrical stimulation and acoustic stimulation. The mechanical vibration stimulation module, the wireless transmission module, the wireless receiving module and the relay driving module are assembled and fixed on the carrier bundled on the outer side of the experimental animal body, the carrier may be an elastic canvas, and the canvas is further provided with a stimulation electrode 4, The specific structure of the carrier is shown in Figures 1-3, and the ends of the canvas are provided with bonding buckles 1, 3 to facilitate binding of the experimental animals, see Figure 9.

The electric stimulation module is mainly composed of a normally open contact Kc controlled by a relay Jc, a triode V5, a transformer T, a resistor R14, R18, an LED D6, diodes D7, D8, D9, capacitors C5, C6, C7 and an electrode DJ;

Mechanical stimulation module: mainly composed of motor M, resistor R 13, and normally open contact Kb controlled by relay Jb;

Acoustic stimulation module: mainly composed of 555 time base circuit IC5, resistor R7, R8, capacitor C3, relay Ja controlled normally open contact Ka, capacitor C4 and speaker Y;

Relay drive module: mainly composed of single chip IC6, relay Ja, Jb, Jc, triode V2, V3, V4, diode D3, D4, D5, resistor R10, Rl l, R12;

Wireless receiving module: mainly composed of wireless remote control receiving head C3, decoding integrated chip IC4, capacitor C8, C9, C10, resistor R9; wireless transmitting module: mainly by wireless remote control transmitter IC1, 555 time base integrated circuit IC2, diode Dl, diode D2, D10, resistor

Rl, R2, R3, R4, R5, R6, R15, R16, R17, potentiometer W, capacitor Cl, capacitor C2, switch Kl, K2, K3 and microcontroller IC7;

Second, the circuit connection structure of the device is as follows, see Figure 4:

Acoustic stimulation module circuit: Capacitor C3 is connected between 555 time base circuit IC5 (NE555) between pins 1 and 2, 555 time base circuit IC5 2 and 6 pins are shorted and then connected to resistor R7, and resistor R7 is connected to 555. Between the 6th and 7th pins of the time base circuit IC5, the resistor R8 is connected to the 4th and 7th pins of the 555 time base circuit IC5, and the 4th and 8th pins of the 555 time base circuit IC5 are shorted and then connected to the relay Ja. The open contact Ka, the capacitor C4 and the speaker Y are connected in series and connected between the 555 time base circuit IC5 and the ground. When the normally open contact Ka controlled by the relay Ja is closed, the speaker Y emits a sound stimulus.

Wireless receiving module circuit: Wireless remote control receiver The positive and negative terminals of IC3 are connected to the positive and negative terminals of power supply E respectively. Radio remote control receiver IC3 negative and speaker ¥, capacitor C3, 555 time base circuit IC5 pin 1, capacitor C8, capacitor C9, capacitor C10, decoding integrated chip IC4 pin 7, transistor V2, V3, V4, The collectors are commonly grounded. Wireless remote control receiver IC3 positive and decoding integrated chip IC4 pin 4 and relay Ja, relay Jb, relay Jc normally open contacts Ka, Kb, Kc and transformer winding L1 connection.

The 3-pin of the decoding integrated chip IC4 is connected to the wireless remote control receiving head IC3. The 1 pin of the decoding integrated chip IC4 is grounded through the capacitor C8, and the 2 pin of the decoding integrated chip IC4 is grounded through the capacitor C9, and the 6 pin of the decoding integrated chip IC4 is grounded through the capacitor C10. The 7 pins of the decoding integrated chip IC4 are directly grounded. The 6 pin of the decoding integrated chip IC4 is grounded through the resistor R9 and the capacitor C10. P3. 1 and P3. 2 of the single chip IC6 are connected to the 8 pin of the integrated chip IC4, and the PL 5 of the single chip IC6 is connected to the base of the transistor V2 through the resistor R10; the P1. 6 of the single chip IC6 passes through the resistor R11 and the transistor V3. Base connection; P1. 7 of single chip IC6 is connected with the base of transistor V4 through resistor R12; the emitter of transistor V2 is connected to relay Ja, relay Ja is anti-parallel diode D3, the emitter of transistor V3 is connected to relay Jb, relay Jb The anti-parallel diode D4, the emitter of the transistor V4 is connected to the relay Jc, and the relay Jc is connected in parallel with the diode D5.

Mechanical vibration stimulation module circuit: The resistors R13 and Kb are connected in series with the motor and connected between the normally open contact Kb of the relay Jb and the ground. When the normally open contact Kb of the relay Jb is closed, the motor M generates mechanical vibration stimulation.

Electrical stimulation module circuit: the collector of the transistor V5 is connected to the switch Kc and the input of the transformer primary winding L1 side, the base of the transistor V5 is connected to the other end of the transformer primary winding L1 through the resistor R18, and the emitter of the transistor V5 is connected. Transformer primary winding L2 - terminal. The transformer side is connected to the diodes D7, D8, D9 and the metal electrode DJ; the diode D7 and the diode D8 are connected in series and connected in parallel with the capacitor C6. Diode D8 and diode D9 are connected in series and connected in parallel with capacitor C7. Diode D8, diode D9, and DJ are connected in series and connected in parallel with capacitor C5. When the normally open contact Kc controlled by the relay Jc is closed, the electrode DJ is electrically stimulated to the experimental animal.

Wireless transmitter module circuit, see Figure 5: +12V regulated power supply is connected to the wireless remote control transmitter IC1 positive, 555 time base circuit IC2 8 pin and resistor Rl. The resistor R1, the potentiometer W and the resistor R2 are connected in series, and the anti-series diode D2 is connected between the 2 pin of the 555 time base circuit IC2 and the +12V regulated power supply. The potentiometer W is connected to the 5 pin of the 555 time base circuit IC2. Diode D1 is connected between the 5 and 6 pins of the 555 time base circuit IC2. 555 Time Base Circuit The 6 and 2 pins of IC2 are grounded through capacitor C1. 555 time base circuit IC2's 1 pin is grounded. The 555 time base circuit IC2's 7 pin is grounded through capacitor C2. The resistor R3 is connected to the 3-pin of the 555 time base circuit IC2 and the IN of the wireless remote control transmitter IC1. Wireless remote control The ground of the IC1's negative terminal.

3. The invention is realized by the setting of the above module: The wireless transmitting module transmits a wireless signal according to the collected door signal of the experimental animal, and after receiving the wireless signal, the wireless receiving module determines the type of the stimulus and activates the relay driving module to implement the corresponding for the experimental animal. Electrical stimulation, acoustic stimulation, mechanical vibration stimulation or combined stimulation.

The specific working principle is as follows: The wireless transmitting module adopts ATS9C51 single chip IC7 to control NE555 type time base integrated circuit IC2, and the light emitting diode D10 is the transmitting indicator light. When the P3.7 pin of AT89C51 single chip IC7 outputs high level, NE555 time base is integrated. The corresponding pin of circuit IC2 is low, the time base integrated circuit IC2 is in reset state, the wireless remote control transmitter IC1 has no signal output, the diode D10 indicator is off; when the P3.7 pin of the single chip IC7 outputs low level, the time base The integrated circuit IC2 is in a normal working state, and the wireless remote control transmitting head IC1 sends N sets of wireless signals with time intervals, and the diode D10 indicator lights, N l ;

The wireless receiving module receives each set of wireless signals by using the wireless remote control receiving head IC3, and the AT89C2051 single chip IC6 of the relay driving module identifies the selected stimulation type according to the number N of wireless signals, and then controls the corresponding relays Ja, Jb, Jc to be constantly open. The head is sucked to achieve acoustic stimulation, vibration stimulation and electrical stimulation or combined stimulation.

The manual switches Kl, Κ2, Κ3 correspond to the acoustic stimulation, vibration stimulation and electrical stimulation respectively. The AT89C51 single-chip IC7 of the wireless transmitting module controls the time base integrated circuit IC2 to send the corresponding number of wireless according to the setting state of the manual switches K1, Κ2, Κ3. signal.

Working process: The transmitting circuit part AT89C51 MCU controls the ΝΕ555 type time base integrated circuit (IC2) to send a set of wireless signals (N1) at a certain interval (for example, 10MS) according to the setting state of Kl, Κ2, Κ3, AT89C2051 The MCU identifies the selected stimulus type according to the number of received wireless signals of each group, controls the corresponding relay to pull in, and realizes the stimulation action. The details are shown in Table 1.

When the experimental animals are tested in the mouse path training model and the intelligent behavior monitoring system, the receiving and stimulating devices bundled on the back of the experimental mouse are composed of a triode V5 and a transformer Τ when the mouse passes through the non-green door. When the oscillator is energized, the power supply Ε DC power is converted into high-frequency alternating current. After being boosted by Τ, it is rectified to high voltage by diodes D7~D9 and capacitors C5~C7 three times, and is applied to the metal electrode DJ. To generate electrical stimulation, the 555 time-base circuit IC5 forms an audible stimulation circuit, and the mechanical vibration is generated by a micro-cell vibration motor. Fourth, the choice of components: transistor V5 selects 8050; V2, V3, V4 select A781. D6, D10 with red LED, D7 ~ D9 with 1N4007 silicon rectifier diode, high-frequency transformer T can be homemade. R13 selects 1W metal film resistor, R14, R18 selects RTX-1/8W type carbon film resistor, the rest selects 1 / 4W carbon film resistor or metal film resistor, RP selects solid variable resistor, C5, C6, C7 Use CL11-630V type polyester capacitor, and the rest use monolithic capacitor. Dl, D2, D3, D4, D5 select 1N4148 type silicon switch diode; IC1 selects T630 type integrated wireless remote control transmitter; IC2 selects E555 type time base integrated circuit; IC3 selects T631 type wireless remote control receiver which is matched with IC1; IC4 The LM567 type decoding integrated circuit is selected. Ja, Jb, and Jc use DC relays. IC5---NE555 type time base integrated circuit; IC6---AT89C2051 single-chip microcomputer; IC7---AT89C51 single-chip microcomputer Y-speaker; M micro-DC motor.

5. Description of a labyrinth apparatus to which the stimulation device of the present invention is applied.

The labyrinth device comprises a circular box 5 and a circuit control system. A plurality of partitions 6 are arranged between the inner wall of the circular box 5 and the central cylinder, thereby dividing the box into a plurality of data collection areas and destination areas, each partition The bottom of the panel 6 is uniformly distributed with the same door opening 17 as the bottom. The improvement point of the labyrinth device is as shown in FIG. 10, the bottom of the box body 5 is provided with a turntable 10, and the central column body is provided with a motor 8, and the output shaft of the motor 8 is fastened and connected to the center of the turntable 10 along the axial direction of the center cylinder, then the turntable 10 The motor 8 can be rotated around the center cylinder. A pulley 16 with a plurality of turntables is arranged around the lower part of the frame of the box body 5, while supporting the turntable, it does not affect its rotation. A sieve-like support net 9 is provided above the turntable 10. The support net 9 can be made of other materials such as metal mesh or plastic. A water tank 12 is formed under the turntable 10 to form a manure collection and processing device. A water pipe 11 is arranged on the water tank 12, and a water spray port is uniformly arranged on the water pipe 11. The bottom of the water tank 12 is provided with a support frame and a water outlet, and the water tank 12 is also provided with water. Brush 13.

The circuit control system includes a power supply, a single chip microcomputer, an olfactory controller, an automatic flushing drainage system controller, and the like. The single chip transmits a signal to the motor 8 of the control turntable 10 to realize the rotary motion of the turntable 10. The motor control of this application is controlled by solid state relay, and the power of the drive motor is <100 _W. In this paper, the solid state relay uses lOOOOw. The microcontroller indirectly controls the relay through the MOS transistor. The instruction of the MCU to control the motor comes from the instruction sent by the host computer through the CAN bus. The CAN and RS232 conversion interface circuits implement data conversion between the CAN bus protocol and the RS232 protocol. The upper computer command is sent to each child node through the system. Automatic flushing drainage system (fecal collection system) The controller receives the AT89S51 single-chip microcomputer P3.6 (I/0) to send out control signals to control the operation of its BFC-2 type electric trick controller, and then control the electric installation on the fecal collection and processing device. The electric valve is opened/closed, so that the water pipe 11 disposed along the water tank 12 of the fecal collecting and processing device is connected or blocked with the water source, and the tap water source is flushed through the electric valve to the sewage device, that is, the water tank, and the sewage flows out from the water outlet. The upper part of the water brush 13 disposed in the water tank 12 is fixed at the bottom of the turntable 10, and the lower brush body is in contact with the side wall of the water tank and the bottom of the tank. When the single-chip microcomputer controls the turntable 10 to rotate, the water brush 13 rotates accordingly, and the lower brush body can be opposite. The water tank 12 is brushed to remove the excrement of the animal, thereby eliminating the influence of the odor on the experimental effect. Odor release device: The MCU controls the LCD LCD and the odor controller simultaneously through the olfactory controller. After detecting the olfactory signal, the MCU transmits the fresh air to the training system through the air compressor, the control valve, the odor generator and the pressure reducing valve. The set arm is then transported by the robot arm to the scent can set in the labyrinth position.

The general structure of the labyrinth device of the present invention, that is, the number of the classic type configurable partitions is 6, and the circular box is equally divided into six sections of A, B, C, D, E, and F, as shown in FIG. There are 5 data collection areas and 1 target area. There are 4 door openings at equal intervals under each partition. The partitions of each section are provided with LCD screens, and the destination area is provided with food slots. Two photoelectric radiation sensor units are arranged at each door hole provided under the partition plate, and the front door of the door is placed separately and outputted in the form of 'or', so that the direction of the rat crossing the door can be detected to detect the walking track of the mouse. Acousto-optic stimulation of the experimental mouse: Through the 10 ports of the single-chip microcomputer, the gate of the MOS tube is controlled to realize the control of the illumination source (liquid crystal panel) and the sound source, and the frequency of the sound generation and the intensity of the illumination can be controlled. Wherein, the single chip microcomputer is used as a sub-receiving unit to receive data information transmitted from the main control unit, from the data letter The command information is extracted from the interest, and then the sound stimulation and the light stimulation are emitted. The control data from which the stimulus can be extracted from the data information has luminous intensity, vocalization frequency, and the like. Visual Stimulation Unit for Experimental Rats: The ViSaGe Visual Stimulation Generator is used in the prior art. ViSaGe uses 14bit DCAs image output technology to ensure that ViSaGe controls the output of the visually stimulating image color, brightness and accurate display time, even when the image is quickly switched, no frame loss occurs. The CRS MATLAB toolkit makes it easier and faster to output visual stimulus images using MATLAB language programming. At the same time, multiple interfaces ensure that ViSaGe can be used in sync with other devices such as ResonseBox, EyeTracker, EEG, Optotrak and MRI. The food trough is a drum type with a mesh on the side, and a bottom surface can be attached to a smooth partition surface. On the other bottom surface opposite thereto, there is an opening for the food trough, and the food can be placed in the food trough by using the upper half of the foldable semicircle. The outer side of the circular side of the food trough 18 is provided with an annular outer casing. The outer casing is divided into two, and the lower part can be separately exposed to the grid, which is controlled by the circuit control system. When the outer casing is opened, the animal can eat food from below the grid of the food trough. As a reward for completing the training task, after a certain period of time (10s), the power is turned off, the jacket is closed, the grid is sealed, and the animal cannot eat the food.

The animal memory training system formed by the above labyrinth device is more ideal, and has great significance for research in the fields of neurophysiology, neuropharmacology and psychology.

Table 1 :

No. The number of signals in this group.

1 1 vocal

2 2 vibration

3 3 electricity

4 4 sound, vibration

5 5 sound, electricity

6 6 Vibration, electricity

7 7 sound, vibration, electricity

Claims

Rights request

A smart wireless remote control stimulation device for an animal memory training system, comprising an electrical stimulation, an acoustic stimulation, and a mechanical vibration stimulation module, further comprising: a wireless transmitting module, a wireless receiving module and a relay driving module, wherein the electrical The stimulation, the acoustic stimulation, the mechanical vibration stimulation module, the wireless transmission module, the wireless receiving module and the relay driving module are assembled and fixed on the carrier bundled outside the body of the experimental animal, and the wireless transmitting module is based on the collected signal of the experimental animal. Transmitting a wireless signal, after receiving the wireless signal, the wireless receiving module determines a stimulus type and activates the relay driving module to implement a corresponding electrical stimulation, acoustic stimulation, mechanical vibration stimulation or combined stimulation on the experimental animal.

2. The intelligent wireless remote control stimulation device for an animal memory training system according to claim 1, wherein: said wireless transmission module comprises a wireless remote control transmitter IC1, a time base integrated circuit IC2, a diode D10, and a potentiometer W. The manual switch K1, Κ2, Κ3 and the single chip IC7; the wireless receiving module comprises a wireless remote control receiving head IC3, a decoding integrated chip IC4; the relay driving module comprises a single chip IC6, relays Ja, Jb, Jc _;

The wireless transmitting module uses a single chip IC7 to control the time base integrated circuit IC2, and the diode D10 is a transmitting indicator light. When the P3.7 pin of the single chip IC7 outputs a high level, the corresponding pin of the time base integrated circuit IC2 is low. The time base integrated circuit IC2 is in a reset state, the wireless remote control transmitting head IC1 has no signal output, the diode D10 indicator light is off; when the P3.7 pin of the single chip IC7 outputs a low level, the time base is integrated. The circuit IC2 is in a normal working state, and the wireless remote control transmitting head IC1 transmits N sets of wireless signals with time intervals, and the diode D10 indicator light is bright, N2=l;

The wireless receiving module receives each set of wireless signals by using the wireless remote control receiving head IC3, and the single chip IC6 of the relay driving module identifies the selected stimulation type according to the number N of wireless signals, and further controls the corresponding relay Ja. , Jb, Jc normally open contacts to achieve stimulation.

3. The intelligent wireless remote control stimulation device for an animal memory training system according to claim 2, wherein: said manual switches K1, Κ2, Κ3 respectively correspond to acoustic stimulation, vibration stimulation and electrical stimulation selection, said wireless The single chip IC7 of the transmitting module controls the time base integrated circuit IC2 to transmit a corresponding number of wireless signals according to the setting states of the manual switches K1, Κ2, and Κ3.

4. The intelligent wireless remote control stimulation device for an animal memory training system according to claim 2, wherein: said mechanical stimulation module comprises a motor Μ, a resistor R13, and a normally open contact Kb controlled by a relay Jb, The resistor R13 and the normally open contact Kb are connected in series with the motor M, and are connected between the normally open contact Kb of the relay Jb and the ground. When the normally open contact Kb of the relay Jb is closed, the motor M generates mechanical vibration stimulation.

5. The intelligent wireless remote control stimulation device for an animal memory training system according to claim 2, wherein: said sound stimulation module comprises a time base circuit IC5, resistors R7, R8, capacitors C3, C4, and relay Ja control. The normally open contact Ka and the speaker Y, the capacitor C3 is connected between the 1 and 2 pins of the time base circuit IC5, and the 2 and 6 pins of the time base circuit IC5 are shorted and then connected to the resistor R7, and the resistor R7 is connected. Between the 6 and 7 pins of the base circuit IC5, the resistor R8 is connected between the 4 and 7 pins of the 555 time base circuit IC5, and the 4 and 8 pins of the time base circuit IC5 are shorted and then connected to the relay Ja controlled normally open touch. At point Ka, the capacitor C4 is connected in series with the speaker Y and connected between the 555 time base circuit IC5 and the ground. When the normally open contact Ka controlled by the relay Ja is closed, the speaker Y emits a sound stimulus.

6. The intelligent wireless remote control stimulation device for an animal memory training system according to claim 2, wherein: said electric The stimulation module includes a normally open contact Kc controlled by a relay Jc, a transistor V5, a transformer T, a resistor R18, an LED D6, a diode D7-D9, a capacitor C5-C7 and an electrode DJ, and the collector of the transistor V5 is normally open. Point Kc and the input end of the primary winding L1 of the transformer, the base of the transistor V5 is connected to the other end of the primary winding L1 of the transformer through the resistor R18, and the emitter of the transistor V5 is connected to the L2- terminal of the primary winding of the transformer, and the secondary side of the transformer is connected in series Diodes D7, D8, D9 and metal electrode DJ, diodes D7, D8 are connected in series with capacitor C6 in series, diodes D8, D9 are connected in series with capacitor C7 in parallel, diodes D8, D9, DJ are connected in series with capacitor C5 in parallel, when the relay When the normally open contact Kc controlled by Jc is closed, the electrode DJ is electrically stimulated to the experimental animal.

The intelligent wireless remote control stimulation device for an animal memory training system according to claim 2, wherein: the wireless receiving module further comprises capacitors C8, C9, CIO, and a resistor R9, wherein the relay driving module further includes Transistor V2, V3, V4, diodes D3, D4, D5, resistors R10, Rl l, R12, the positive and negative poles of the wireless remote control receiving head IC3 of the wireless receiving module are respectively connected to the positive and negative poles of the power source E, the wireless The negative pole of the remote control receiving head IC3 and the speaker Y of the acoustic stimulation module, the capacitor C3, the pin of the time base circuit IC5, the capacitor C8, the capacitor C9, the capacitor C10, the pin 7 of the decoding integrated chip IC4, the relay The collectors of the triodes V2, V3, and V4 of the driving module are commonly grounded, the positive pole of the wireless remote receiving head IC3 and the pin 4 of the decoding integrated chip IC4, the relay Ja, the relay Jb, the normally open contact of the relay Jc, the relay Ja, a relay Jb, a relay Jc coil and a transformer winding L1 of the electrical stimulation module are connected;

Wherein, the 3-pin of the decoding integrated chip IC4 is connected to the wireless remote control receiving head IC3, and the 1 pin of the decoding integrated chip IC4 is grounded through the capacitor C8, and the 2 pins of the decoding integrated chip IC4 are grounded through the capacitor C9, and the integrated chip is decoded. The 6-pin of IC4 is grounded through capacitor C10, and the 7-pin of the decoding integrated chip IC4 is directly grounded. The 6-pin of the integrated integrated chip IC4 is grounded through the resistor W and the capacitor C10, and the P3. 1 and P3.2 of the microcontroller IC6 are connected. Decode the integrated chip IC4 pin 8; the microcontroller IC6 P1. 5 through the resistor R10 and the base of the transistor V2, PL6 of the microcontroller IC6 through the resistor R11 and the base of the transistor V3, the microcontroller IC6 P1. 7 through the resistor R12 is connected to the base of transistor V4; the emitter of transistor V2 is connected to relay Ja, relay Ja is anti-parallel diode D3, the emitter of transistor V3 is connected to relay Jb, relay Jb is anti-parallel diode D4, and the emitter of transistor V4 is connected in series. Relay Jc, relay Jc anti-parallel diode D5.

8. The intelligent wireless remote control stimulation device for an animal memory training system according to claim 2, wherein: said wireless transmitting module comprises a capacitor C1, C2, a diode D1, D2, a resistor Rl-R6, and a R15-R17. , +12V regulated power supply is connected to the wireless remote control transmitter IC1 positive, 555 time base circuit IC2 8 pin and resistor Rl, the resistor Rl, potentiometer W and resistor R2 are connected in series, and the anti-series diode D2 is connected to the time base. Between the 2-pin of the circuit IC2 and the +12V regulated power supply, the potentiometer W is connected to the 5 pin of the time base circuit IC2, and the diode D1 is connected between the 5th and 6th pins of the time base circuit IC2, and the time base circuit IC2 The 6 pin and the 2 pin are grounded through the capacitor C1, the 1 pin of the time base circuit IC2 is grounded, the 7 pin of the time base circuit IC2 is grounded through the capacitor C2, and the resistor R3 is connected to the 3 pin of the time base circuit IC2 and the wireless remote control. The IN of the transmitting head IC1, the negative pole of the wireless remote control transmitting head IC1 is grounded.

9. The intelligent wireless remote control stimulation device for an animal memory training system according to claim 1, wherein: the carrier bundled on the outside of the body of the experimental animal is an elastic canvas, and the canvas is further provided with a stimulation electrode. The two ends of the canvas are provided with a fastening buckle.