WO2012047081A1 - Proceso de elaboración de granulos peletizados a base de hongos endomicorrízicos recubiertos con arcillas minerales y su composición - Google Patents
Proceso de elaboración de granulos peletizados a base de hongos endomicorrízicos recubiertos con arcillas minerales y su composición Download PDFInfo
- Publication number
- WO2012047081A1 WO2012047081A1 PCT/MX2010/000116 MX2010000116W WO2012047081A1 WO 2012047081 A1 WO2012047081 A1 WO 2012047081A1 MX 2010000116 W MX2010000116 W MX 2010000116W WO 2012047081 A1 WO2012047081 A1 WO 2012047081A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- spores
- mixture
- composition according
- endomicorrhizal
- composition
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/40—Fertilisers incorporated into a matrix
Definitions
- the present invention relates to a process for obtaining a composition in the form of pelletized granules based on spores of endomicorrhizal fungi coated with mineral clays and the composition thus obtained.
- This coating serves as an armor for the spores of endomicorrhizal fungi because it protects them from adverse environmental conditions and gives them resistance to handling, preventing their destruction due to mechanical damage that could result during their handling, also protects them against drastic changes in temperature avoiding his dehydration and / or death.
- Fine sand 0.25-0.10 0.20-0.02 Very fine sand 0.10-0.05 0.02-0.002
- a soil For a soil to be designated as a clay, it must have at least 35% clay fraction.
- the clay exists in the so-called colloidal state, in which its individual particles are characterized by their extraordinarily small size, large area of dispersion per unit mass and the presence, on the surface, of electrical charges to which both molecules are attracted of water like ions.
- the clay particles are currently arranged in plates or scales, such as mica, and if they get wet they are very plastic. When the clay is wetted with an adequate amount of water, it expands and becomes sticky. On drying it shrinks with considerable energy absorption. Wetting again, swelling appears, with temperature change. The absorbent capacity of clays for water, gases and soluble salts is very high.
- Three types of important minerals are recognized, although others are known in significant quantities, kaolinite, Hita and montmorillonite. These groups vary greatly in plasticity, cohesion and adsorption, being the kaolinite the lowest in these properties and the montmorillonite the highest.
- kaolinite and the other members of this special group are aluminum silicates. The same happens with montmorillonite and other clays of the same type of crystallization, but these also carry sodium, iron or magnesium, depending on the case.
- a bentonite is a rock composed essentially of minerals from the smectite group. The classification criteria used by the industry are based on their behavior and physical-chemical properties; Thus, the most accepted industrial classification establishes three types of bentonites according to their capacity for water laundering:
- the physicochemical properties of clays derive, mainly from:
- the high plasticity of the clays is a consequence, again, of their laminar morphology, extremely small particle size (high surface area) and high swelling capacity.
- biofertilizers that is, products of natural occurrence, whose active ingredient is the spores or vegetative cells of microorganisms, or plant extracts; It is a useful tool to approach sustainable agriculture.
- mycorrhizae which are beneficial associations that are established between some soil fungi and the roots of vascular plants.
- the mycorrhizae improve the growth of the plant by increasing the surface of absorption of the root system; by selectively absorbing and accumulating certain nutrients, especially phosphorus; by solubilizing and making some minerals available to the plant normally insoluble; by allowing feeder roots to work longer; and by making the feeder roots more resistant to infection caused by some pathogenic fungi to the ground such as Phytophthora, Pythium and Fusarium.
- endimicorrhizal fungi are They are fully exposed to environmental conditions and mechanical damage during product handling, suffering structural breakage or even death, which significantly decreases the effectiveness of the product.
- endimicorrhizal fungi are exposed, there is a risk that they will come into direct contact with some fungicidal product that has also been applied to the seed causing death.
- fungi are susceptible to being parasitized by some harmful agent preventing their installation at the root of the plants and / or causing their death.
- the present invention relates to a process for obtaining a composition in the form of pelletized granules based on spores of endomicorrhizal fungi coated with mineral clays and the composition thus obtained as well as its use in agriculture.
- the composition it comprises: a) a mixture of endomicorrhizal fungal spores and sterilized slime in a proportion of 15% to 30% by weight; b) mixture of mineral clays in a proportion of 58% to 75% by weight and c) binder in a proportion of 10 to 12% by weight.
- the mixture of endomicorrhizal fungal spores with sterile slime contains a spore concentration of 0-05% to 0.10%, this concentration allows the final pelletized product to contain up to 3,000 viable spores / Kg, an amount that ensures efficient use of the product.
- This mixture must contain at least one of the following species of endomicorrhizal fungi: Glomus fasciculatum, Glomus constrictum, Glomus tortuosum, Glomus geosporum, Glomus intraradices, and / or mixtures of these.
- composition of the mixture of montmorillonite, kaolinite and bentonite mineral clays is 0.5: 0.5: 1.
- the particle size of the components of the mixture plays an important role in achieving proper homogenization and granule formation.
- the sterile slime mixture with the spores of endomicorrhizal fungi must contain particles whose size must be at least 40 mesh (0.425mm).
- the particle size of the components of the clay mixture must be between 80 mesh (0.180 mm) and 100 mesh (0.150 mm).
- Another embodiment of the invention consists in the process to obtain a composition based on spores and mineral clays for use in agriculture comprising the following steps: i) The clay mixture is introduced into a hopper Mixer together with the sterilized slime containing the spores of the endomicorrhizal fungi and the binder, in this first stage a dry mixture of all the ingredients is carried out. At this point the clays come into contact with the spores of the endomicorrhizal fungi and the covering process begins.
- the use of a binder is essential to give the granule a greater hardness. This mixture maintains the components in the proportions and ranges described above until they are completely homogenized.
- the temperature is reduced to a range between 200 to 300 ° C and in the last section the temperature range decreases to 80 and 130 ° C.
- the granules obtained are passed through a screening system where the largest opening mesh is number 3 (5 mm) and the smallest is number 20 (0.85mm).
- the product that is retained between these meshes is appropriate and is packaged in sacks or paper bags. This is done in order to ensure that the product to be delivered meets the optimum granulometry conditions for easy application.
- a further embodiment of the invention is that the mixing of the components subject to the process described above removes water from the final pelletized product in order to obtain a low moisture concentration between 2% and 6%, which allows it to be also mixed with hygroscopic fertilizers such as urea, without problems that may affect its physical characteristics.
- another embodiment of the invention consists in the use of binders in the mixture to obtain the granulated product. Tests were carried out with different binders such as: hydrated lime, molasses, pectins, calcium sulphonate and calcium sulfate monohydrate; and according to the results obtained, the binder with the best results is calcium sulfate monohydrate.
- another embodiment of the invention is that the use of the binder gives the dry granulated product properties of adequate hardness that will give it resistance against mechanical damage by handling and storage.
- the dry granulated product has a hardness between 1.9 to 2.3 kg / cm 2. This hardness is measured with a penetrometer or durometer.
- the granulated product made from spores of endomicorrhizal fungi that are covered by a mixture of mineral clays (montmorillonites, kaolinites and bentonites) referred to in the present invention is a versatile product of easy and practical application. Its presentation is in granular form, with a particle size of between 0.85 to 5.0 millimeters, appropriate size that allows it to be easily applied either alone or in admixture with others fertilizers It has a hardness between 1.9 to 2.3 kg / cm 2 , enough to withstand handling during product preparation and subsequent mixing with other fertilizers.
- the spores of the endomicorrhizal fungi are covered by a mixture of mineral clays (montmorillonites, kaolinites and bentonites) and then they are subjected to a palletizing process (granulation), this protects them against the mechanical damage that It could be presented during product handling making each application more effective.
- the spores are also protected against drastic changes in temperature, or against the infection of a parasite or the contamination of some harmful agent, preventing their destruction and / or death.
- This granulated product can be applied alone or in admixture with fertilizers directly to the soil, as it is not applied to the seed, it is not necessary to use any adherent or any kind of carrier or carrier additives. Examples
- the formed granules are fed to a three section rotary kiln with a temperature profile of 450, 225 and 100 ° C for each of the sections and obtain a final product moisture of 5%. After drying, the granules are screened through the 0.85 mm (20 mesh) and 5.0 mm (3 mesh) opening meshes. A granulated product with a hardness of 2.0 kg / cm 2 was obtained . The granulated product obtained can be applied in doses of 10-30 Kg / ha in any crop, alone or in a mixture with the fertilizer, in soils where the established crops meet low fertility levels.
- a process for the selection of temperatures at which the granulation process will be carried out was carried out. For this, 4 temperatures were chosen at which the oven operates. These temperatures were selected based on the drying processes that were already available for drying other products based on mineral clays. The temperatures chosen for the experimentation were: 300, 500, 600 and 900 ° C. The output response evaluated was the viability of the product expressed in # of viable spores / kg of sample.
- This figure also shows the separation of spores, also in Figure 2. a) Spore considered viable with characteristic color and turgidity, in Figure 2 b) Spore considered dead by the color it presents and without turgidity and figure 2 c) Spore considered not viable due to cell wall rupture.
- the effect of the granulated product based on spores of endomicorrhizal fungi in wheat plants was evaluated. Plants with granulated mycorrhizae and other plants with clay without granules were planted in pots. First, the spore counts contained in the applied dose were adjusted to adjust the concentration per pot, so that the concentration of 30,000 spores / hectare was met.
- Figure 1 is a graph, which represents the evaluation of drying temperature vs Viability, of viable spores / kilogram, where it is observed that the viability decreases as the drying temperature increases.
- Figure 2 represents a photograph of the spore separation.
- Figure 2 a) represents a spore considered viable with characteristic color and turgidity.
- Figure 2 b represents a spore considered dead by the color it presents and without turgidity.
- Figure 2 c represents a spore considered not viable due to cell wall rupture.
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BRPI1007087A BRPI1007087A2 (pt) | 2010-10-04 | 2010-10-25 | "processo de elaboração de grânulos peletizados à sua base de fungos endomicorrízicos revestidos com argilas minerais e sua composição" |
US13/581,280 US8993482B2 (en) | 2010-10-04 | 2010-10-25 | Process to make pelletized granules based on endomycorrhizal fungi covered with minerals clays and their composition |
US14/566,549 US9681667B2 (en) | 2010-10-04 | 2014-12-10 | Process to make pelletized granules based on endomycorrhizal fungi covered with minerals clays and their composition |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MXMX/A/2010/010894 | 2010-10-04 | ||
MX2010010894A MX2010010894A (es) | 2010-10-04 | 2010-10-04 | Proceso de elaboracion de granulos peletizados a base de hongos endomicorrizicos recubiertos con arcillas minerales y su composicion. |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US13/581,280 A-371-Of-International US8993482B2 (en) | 2010-10-04 | 2010-10-25 | Process to make pelletized granules based on endomycorrhizal fungi covered with minerals clays and their composition |
US14/566,549 Division US9681667B2 (en) | 2010-10-04 | 2014-12-10 | Process to make pelletized granules based on endomycorrhizal fungi covered with minerals clays and their composition |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2012047081A1 true WO2012047081A1 (es) | 2012-04-12 |
Family
ID=44996558
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/MX2010/000116 WO2012047081A1 (es) | 2010-10-04 | 2010-10-25 | Proceso de elaboración de granulos peletizados a base de hongos endomicorrízicos recubiertos con arcillas minerales y su composición |
Country Status (9)
Country | Link |
---|---|
US (2) | US8993482B2 (es) |
AR (1) | AR083157A1 (es) |
BR (1) | BRPI1007087A2 (es) |
CL (1) | CL2013000902A1 (es) |
CO (1) | CO6690797A2 (es) |
EC (1) | ECSP12012150A (es) |
GT (1) | GT201300086A (es) |
MX (1) | MX2010010894A (es) |
WO (1) | WO2012047081A1 (es) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103342601A (zh) * | 2013-07-09 | 2013-10-09 | 黑龙江久胜生物科技有限公司 | 生物控释肥料 |
WO2015199541A1 (en) | 2014-06-26 | 2015-12-30 | Ecostyle B.V. | Fertilizer comprising bacteria and protozoa. |
WO2017105238A1 (en) | 2015-12-17 | 2017-06-22 | Ecostyle B.V. | Fertilizer comprising bacteria and protozoa. |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4551165A (en) * | 1982-04-22 | 1985-11-05 | National Research Development Corporation | Mycorrhizal seed pellets |
EP0485229B1 (en) * | 1990-11-08 | 1995-01-18 | Shell Internationale Researchmaatschappij B.V. | Water-dispersible granules comprising va mycorrhizal fungi, their preparation and use |
US20080064598A1 (en) * | 2004-11-04 | 2008-03-13 | Mycosym International Ag | Mycorrhizal Fungi Preparation, their Production and their Use in the Inoculation of Plants |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4316813A (en) * | 1980-08-04 | 1982-02-23 | Engelhard Minerals & Chemicals Corp. | Limestone-based sorbent agglomerates for removal of sulfur compounds in hot gases and method of making |
DE3416315A1 (de) | 1984-05-03 | 1985-11-07 | Chemische Werke Hüls AG, 4370 Marl | Herstellung und verwendung von adsorbentien zur inokulation von pflanzen mit vesikulaer-arbuskulaeren mykorrhizapilzen |
EP0314439A3 (en) * | 1987-10-26 | 1989-11-08 | Native Plants Incorporated | Microbial inoculants and methods for producing same |
DE3932746A1 (de) | 1989-09-30 | 1991-04-11 | Weritz Juergen Dipl Agr Ing | Traegermaterial fuer die produktion und applikation von va-mykorrhizapilzpropagationsorganen sowie verfahren fuer dessen weiterverarbeitung |
US5212144A (en) * | 1992-06-01 | 1993-05-18 | Westvaco Corporation | Process for making chemically activated carbon |
AU2002348626A1 (en) * | 2002-10-16 | 2004-05-04 | Mezclas Y Fertilizantes S.A. De C.V. | Granulated fertiliser comprising micronutrients and clay |
-
2010
- 2010-10-04 MX MX2010010894A patent/MX2010010894A/es active IP Right Grant
- 2010-10-25 US US13/581,280 patent/US8993482B2/en active Active - Reinstated
- 2010-10-25 BR BRPI1007087A patent/BRPI1007087A2/pt not_active IP Right Cessation
- 2010-10-25 WO PCT/MX2010/000116 patent/WO2012047081A1/es active Application Filing
-
2011
- 2011-09-14 AR ARP110103351A patent/AR083157A1/es not_active Application Discontinuation
-
2012
- 2012-09-12 EC ECSP12012150 patent/ECSP12012150A/es unknown
-
2013
- 2013-04-03 GT GT201300086A patent/GT201300086A/es unknown
- 2013-04-04 CL CL2013000902A patent/CL2013000902A1/es unknown
- 2013-04-10 CO CO13093395A patent/CO6690797A2/es unknown
-
2014
- 2014-12-10 US US14/566,549 patent/US9681667B2/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4551165A (en) * | 1982-04-22 | 1985-11-05 | National Research Development Corporation | Mycorrhizal seed pellets |
EP0485229B1 (en) * | 1990-11-08 | 1995-01-18 | Shell Internationale Researchmaatschappij B.V. | Water-dispersible granules comprising va mycorrhizal fungi, their preparation and use |
US20080064598A1 (en) * | 2004-11-04 | 2008-03-13 | Mycosym International Ag | Mycorrhizal Fungi Preparation, their Production and their Use in the Inoculation of Plants |
Non-Patent Citations (1)
Title |
---|
HALL I R: "Soil pellets to introduce vesicular-arbuscular mycorrhizal fungi into soil", SOIL BIOLOGY AND BIOCHEMISTRY, vol. 11, no. 1, 1979, pages 85 - 86 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103342601A (zh) * | 2013-07-09 | 2013-10-09 | 黑龙江久胜生物科技有限公司 | 生物控释肥料 |
WO2015199541A1 (en) | 2014-06-26 | 2015-12-30 | Ecostyle B.V. | Fertilizer comprising bacteria and protozoa. |
WO2017105238A1 (en) | 2015-12-17 | 2017-06-22 | Ecostyle B.V. | Fertilizer comprising bacteria and protozoa. |
Also Published As
Publication number | Publication date |
---|---|
CO6690797A2 (es) | 2013-06-17 |
US20130196850A1 (en) | 2013-08-01 |
ECSP12012150A (es) | 2012-10-30 |
US8993482B2 (en) | 2015-03-31 |
CL2013000902A1 (es) | 2014-04-04 |
AR083157A1 (es) | 2013-02-06 |
GT201300086A (es) | 2014-10-07 |
US20150296805A1 (en) | 2015-10-22 |
BRPI1007087A2 (pt) | 2016-10-11 |
US9681667B2 (en) | 2017-06-20 |
MX2010010894A (es) | 2011-07-11 |
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